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murine myoblast cells c2c12 human prostate adenocarcinoma cell lines  (ATCC)


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    ATCC murine myoblast cells c2c12 human prostate adenocarcinoma cell lines
    Murine Myoblast Cells C2c12 Human Prostate Adenocarcinoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+myoblast+cell+line+c2c12/MDA+PCa+2b/pm42176796-212-20-44
    Average 96 stars, based on 311 article reviews
    murine myoblast cells c2c12 human prostate adenocarcinoma cell lines - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: Engineering novel AAV capsids by broadly attenuated and subsequent muscle-specific tropism in mice and NHPs
    Article Snippet: 1PackGene Biotech Inc, Houston, TX 77054, USA; 2Laboratory of Regenerative Medicine in Sports Science, School of Physical Education and Sports Science, South China Normal University, Guangzhou 510006, China; 3Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China; 4Department of Genetic and Cellular Medicine, Horae Gene Therapy Center, Li Weibo

    Article Title: Engineering novel AAV capsids by global de-targeting and subsequent muscle-specific tropism in mice and NHPs
    Article Snippet: .. The human embryonic kidney cell line HEK 293T (American Type Culture Collection (ATCC), CRL-11268), human myoblast cell line C2C12 (ATCC, CRL-1772), human cervical carcinoma cell line HelaRC32 (ATCC, CRL-2972), and human hepatoma HuH-7 cell line (National Collection of Authenticated Cell Cultures, SCSP-526) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, C11995500BT) supplemented with 10% fetal bovine serum (FBS; Excell Bio, FSP500) and 1% penicillin-streptomycin (P/S; Biosharp, BL505A) and the hamster ovary cell line CHO-K1 (Cobioer Gene Technology Co., CBP60296) was maintained in F12K media (Sigma-Aldrich, N3520-10X1L) supplemented with 10% FBS and 1% P/S. ..

    Modification:

    Article Title: Engineering novel AAV capsids by broadly attenuated and subsequent muscle-specific tropism in mice and NHPs
    Article Snippet: 1PackGene Biotech Inc, Houston, TX 77054, USA; 2Laboratory of Regenerative Medicine in Sports Science, School of Physical Education and Sports Science, South China Normal University, Guangzhou 510006, China; 3Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China; 4Department of Genetic and Cellular Medicine, Horae Gene Therapy Center, Li Weibo

    Article Title: Engineering novel AAV capsids by global de-targeting and subsequent muscle-specific tropism in mice and NHPs
    Article Snippet: .. The human embryonic kidney cell line HEK 293T (American Type Culture Collection (ATCC), CRL-11268), human myoblast cell line C2C12 (ATCC, CRL-1772), human cervical carcinoma cell line HelaRC32 (ATCC, CRL-2972), and human hepatoma HuH-7 cell line (National Collection of Authenticated Cell Cultures, SCSP-526) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, C11995500BT) supplemented with 10% fetal bovine serum (FBS; Excell Bio, FSP500) and 1% penicillin-streptomycin (P/S; Biosharp, BL505A) and the hamster ovary cell line CHO-K1 (Cobioer Gene Technology Co., CBP60296) was maintained in F12K media (Sigma-Aldrich, N3520-10X1L) supplemented with 10% FBS and 1% P/S. ..



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    shRNA mediated knockdown of Hmgcr in <t>C2C12</t> myoblasts. (A) qPCR was performed in triplicate for each sample with n=5 experiments. Transcript levels were normalized against Gapdh with fold change calculated as Hmgcr knockdown versus scrambled control. Unpaired t-test results are shown. ***, p < 0.0002. (B) shRNA mediated knockdown of Hmgcr in C2C12 myoblasts. Successful shRNA transfection was confirmed by detecting GFP positive cells.
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    shRNA mediated knockdown of Hmgcr in <t>C2C12</t> myoblasts. (A) qPCR was performed in triplicate for each sample with n=5 experiments. Transcript levels were normalized against Gapdh with fold change calculated as Hmgcr knockdown versus scrambled control. Unpaired t-test results are shown. ***, p < 0.0002. (B) shRNA mediated knockdown of Hmgcr in C2C12 myoblasts. Successful shRNA transfection was confirmed by detecting GFP positive cells.
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    shRNA mediated knockdown of Hmgcr in C2C12 myoblasts. (A) qPCR was performed in triplicate for each sample with n=5 experiments. Transcript levels were normalized against Gapdh with fold change calculated as Hmgcr knockdown versus scrambled control. Unpaired t-test results are shown. ***, p < 0.0002. (B) shRNA mediated knockdown of Hmgcr in C2C12 myoblasts. Successful shRNA transfection was confirmed by detecting GFP positive cells.

    Journal: bioRxiv

    Article Title: Effects of HMGCR deficiency on skeletal muscle development

    doi: 10.1101/2024.05.06.591934

    Figure Lengend Snippet: shRNA mediated knockdown of Hmgcr in C2C12 myoblasts. (A) qPCR was performed in triplicate for each sample with n=5 experiments. Transcript levels were normalized against Gapdh with fold change calculated as Hmgcr knockdown versus scrambled control. Unpaired t-test results are shown. ***, p < 0.0002. (B) shRNA mediated knockdown of Hmgcr in C2C12 myoblasts. Successful shRNA transfection was confirmed by detecting GFP positive cells.

    Article Snippet: C2C12 myoblasts were transfected with either a cocktail of three different shRNAs targeting mouse- Hmgcr or scrambled shRNA control ( GeneCopoeia ) using Lipofectamine 3000 ( Invitrogen ).

    Techniques: shRNA, Knockdown, Control, Transfection

    The oxygen consumption rate (OCR) of Hmgcr shRNA versus scrambled shRNA C2C12 myoblasts was determined using the RESIPHER system at (A) 24 hours, (B), 48 hours, and (C) 72 hours. The OCR of the Hmgcr knockdown cells was increased compared to scrambled controls at 24 and 48 hours. (D) Rotenone treatment at 72 hours confirms that the increased oxygen consumption occurred via mitochondrial respiration. (E) A A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at 72 hours shows that the total cellular metabolic activity is higher in Hmgcr knockdown cells compared to scrambled controls. Paired t-tests were performed. *, p <0.05. Error bars show standard error of the mean (SEM).

    Journal: bioRxiv

    Article Title: Effects of HMGCR deficiency on skeletal muscle development

    doi: 10.1101/2024.05.06.591934

    Figure Lengend Snippet: The oxygen consumption rate (OCR) of Hmgcr shRNA versus scrambled shRNA C2C12 myoblasts was determined using the RESIPHER system at (A) 24 hours, (B), 48 hours, and (C) 72 hours. The OCR of the Hmgcr knockdown cells was increased compared to scrambled controls at 24 and 48 hours. (D) Rotenone treatment at 72 hours confirms that the increased oxygen consumption occurred via mitochondrial respiration. (E) A A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at 72 hours shows that the total cellular metabolic activity is higher in Hmgcr knockdown cells compared to scrambled controls. Paired t-tests were performed. *, p <0.05. Error bars show standard error of the mean (SEM).

    Article Snippet: C2C12 myoblasts were transfected with either a cocktail of three different shRNAs targeting mouse- Hmgcr or scrambled shRNA control ( GeneCopoeia ) using Lipofectamine 3000 ( Invitrogen ).

    Techniques: shRNA, Knockdown, MTT Assay, Activity Assay