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murine myoblast cells c2c12 human prostate adenocarcinoma cell lines  (ATCC)


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    ATCC murine myoblast cells c2c12 human prostate adenocarcinoma cell lines
    Murine Myoblast Cells C2c12 Human Prostate Adenocarcinoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+myoblast+cell+line+c2c12/MDA+PCa+2b/pm42176796-212-20-44
    Average 96 stars, based on 311 article reviews
    murine myoblast cells c2c12 human prostate adenocarcinoma cell lines - by Bioz Stars, 2026-09
    96/100 stars

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    shRNA mediated knockdown of Hmgcr in <t>C2C12</t> myoblasts. (A) qPCR was performed in triplicate for each sample with n=5 experiments. Transcript levels were normalized against Gapdh with fold change calculated as Hmgcr knockdown versus scrambled control. Unpaired t-test results are shown. ***, p < 0.0002. (B) shRNA mediated knockdown of Hmgcr in C2C12 myoblasts. Successful shRNA transfection was confirmed by detecting GFP positive cells.
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    shRNA mediated knockdown of Hmgcr in C2C12 myoblasts. (A) qPCR was performed in triplicate for each sample with n=5 experiments. Transcript levels were normalized against Gapdh with fold change calculated as Hmgcr knockdown versus scrambled control. Unpaired t-test results are shown. ***, p < 0.0002. (B) shRNA mediated knockdown of Hmgcr in C2C12 myoblasts. Successful shRNA transfection was confirmed by detecting GFP positive cells.

    Journal: bioRxiv

    Article Title: Effects of HMGCR deficiency on skeletal muscle development

    doi: 10.1101/2024.05.06.591934

    Figure Lengend Snippet: shRNA mediated knockdown of Hmgcr in C2C12 myoblasts. (A) qPCR was performed in triplicate for each sample with n=5 experiments. Transcript levels were normalized against Gapdh with fold change calculated as Hmgcr knockdown versus scrambled control. Unpaired t-test results are shown. ***, p < 0.0002. (B) shRNA mediated knockdown of Hmgcr in C2C12 myoblasts. Successful shRNA transfection was confirmed by detecting GFP positive cells.

    Article Snippet: C2C12 myoblasts were transfected with either a cocktail of three different shRNAs targeting mouse- Hmgcr or scrambled shRNA control ( GeneCopoeia ) using Lipofectamine 3000 ( Invitrogen ).

    Techniques: shRNA, Knockdown, Control, Transfection

    The oxygen consumption rate (OCR) of Hmgcr shRNA versus scrambled shRNA C2C12 myoblasts was determined using the RESIPHER system at (A) 24 hours, (B), 48 hours, and (C) 72 hours. The OCR of the Hmgcr knockdown cells was increased compared to scrambled controls at 24 and 48 hours. (D) Rotenone treatment at 72 hours confirms that the increased oxygen consumption occurred via mitochondrial respiration. (E) A A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at 72 hours shows that the total cellular metabolic activity is higher in Hmgcr knockdown cells compared to scrambled controls. Paired t-tests were performed. *, p <0.05. Error bars show standard error of the mean (SEM).

    Journal: bioRxiv

    Article Title: Effects of HMGCR deficiency on skeletal muscle development

    doi: 10.1101/2024.05.06.591934

    Figure Lengend Snippet: The oxygen consumption rate (OCR) of Hmgcr shRNA versus scrambled shRNA C2C12 myoblasts was determined using the RESIPHER system at (A) 24 hours, (B), 48 hours, and (C) 72 hours. The OCR of the Hmgcr knockdown cells was increased compared to scrambled controls at 24 and 48 hours. (D) Rotenone treatment at 72 hours confirms that the increased oxygen consumption occurred via mitochondrial respiration. (E) A A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at 72 hours shows that the total cellular metabolic activity is higher in Hmgcr knockdown cells compared to scrambled controls. Paired t-tests were performed. *, p <0.05. Error bars show standard error of the mean (SEM).

    Article Snippet: C2C12 myoblasts were transfected with either a cocktail of three different shRNAs targeting mouse- Hmgcr or scrambled shRNA control ( GeneCopoeia ) using Lipofectamine 3000 ( Invitrogen ).

    Techniques: shRNA, Knockdown, MTT Assay, Activity Assay